MMPs in gene- and protein-expression

IL-23 Gene and Protein Expression in Childhood Asthma

Background: Asthma is the chronic inflammation of airways characterized by eosinophilic infiltration, mucus overproduction, airway hyper-responsiveness and airway remodeling. These changes are induced mostly by cytokines which are produced by T helper (Th) 2 cells. Recently, the role of interleukin-23 (IL-23) in the pathogenesis of adultallergic asthma has been studied. Objective: To explore IL...

Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli

Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...

TrKB and FNDC5 Gene Expression and SG6K1 Protein Response to Eccentric and Concentric Resistance Activity in Young Men

Background: The aim of the present study was to investigate the expression of TrKB, FNDC5 and SG6K1 protein in response to eccentric and concentric resistance training in healthy subjects. Methods: 10 healthy men were randomly assigned to two groups of concentric and eccentric contractions of 5 individuals each. Isokinetic contraction protocols included eccentric and concentric knee extension ...

The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis

Advances and challenges in recombinant protein expression in Escherichia coli